Published in Nature Methods

Microscopy analysis, reimagined for the cloud

Open-source platform that brings powerful segmentation, machine learning, and quantitative analysis to your microscopy data — all from your browser.

NimbusImage app showing cell segmentation with GFP fluorescence overlay

Cloud-native

Access your work from anywhere. Analyze vast datasets with advanced machine learning — no installation, no local compute limits, just your browser.

Interactive

Put your hands on your data in completely new ways. Intuitive tools make complex image analysis accessible and — dare we say — enjoyable.

Open source

Our code is freely available for scrutiny, modification, and improvement. Run your own server, contribute to the project, or build on top of it.

What can NimbusImage do?

01

Automatic segmentation

Segment cells, nuclei, and structures with Cellpose, Piscis, and SAM. Fine-tune results with intuitive manual tools.

02

Spot detection

Detect and quantify fluorescent spots with high accuracy — perfect for RNA FISH, protein aggregates, and spatial analysis.

03

Tracking over time

Track cells and objects across time-lapse images. Maintain identity through divisions for lineage tracing and cell cycle analysis.

04

Quantitative measurement

Measure morphology, fluorescence intensity, and spatial relationships. Analyze distributions across experimental conditions.

05

3D & multichannel

Process 3D stacks and multiple fluorescent channels. Investigate colocalization, proximity, and interactions between components.

06

Batch analysis & ML

Process large datasets with automated workflows. Integrate machine learning models or train new ones on your own data.

Nature Methods
Published

Built by scientists, for scientists

NimbusImage was developed over 15 years in the Raj Lab at the University of Pennsylvania in collaboration with Kitware, leaders in open-source scientific software. Read about the platform in Nature Methods.

Built for how you work

Whether you're a solo researcher or running an imaging core, there's a plan that fits.

Individual
Full access to NimbusImage on your own terms. Monthly or yearly options for independent researchers, postdocs, and students.
Get started
Institutional
Flat-fee licensing for research centers based on user count and storage. Scale effortlessly across your entire institution.
Contact us
Work in your browser — or download the app

NimbusImage runs in any modern browser, but we also provide a standalone desktop app that isolates your workspace and delivers better performance for intensive sessions.

Dedicated performance

Isolate NimbusImage from browser tabs for smoother rendering on large datasets.

Cross-platform

Native builds for Windows, macOS (Apple Silicon), and Linux.

Always in sync

Same cloud account, same data. Switch between browser and app seamlessly.

Transparency at the core

NimbusImage is built on open-source principles. Our core technology is developed transparently, and the commercial deployment through CytoPixel Software provides a stable, supported experience.

We are deeply committed to creating accessible, robust tools that help researchers unlock the full potential of their imaging data. Our primary goal is to create tools that assist and delight.

The Raj Lab

University of Pennsylvania. 15+ years of molecular imaging research powering NimbusImage.

Kitware

Industry leaders in open-source software. Developers of Girder and ITK.

CytoPixel Software

Commercial deployment and support for the NimbusImage platform.

Ready to see your data differently?

Sign up and start analyzing in minutes. No installation, no credit card.